實驗室名稱：應用微生物實驗室

實驗室負責人簡介

高旻天，教授，東方學者，泰山學者，博士/碩士生導師。2006年博士畢業于日本大分大學環境工學專業。2006-2007年，任日本學術振興會外國人特別研究員；2006-2007年，任日本大分大學客座研究員；2007-2010年，任豐田汽車公司中央研究所生物研究室研究員；2010-2013年，任日本産業技術綜合研究所生物煉制中心特別研究員；2013-至今；任上海大學生命科學學院教授，東方學者。學術兼職：山東省泰山學者、山東省科學院能源研究所客座研究員兼學術帶頭人、日本化學工學會會員、日本能源學會生物質部會會員。主要從事微生物學、發酵工學、分離工學及代謝工學等方面研究。特别在木質纖維素生物質的預處理、纖維素酶的生産、有機酸、醇發酵及分離等研究領域取得了多項創新性成果。已發表研究論文50餘篇，其中SCI 30餘篇；申請國内外專利10餘項；主持國内外研究項目10餘項。2015年被評選為上海大學 “我心目中的好導師”。日本化學工學會會員、日本能源學會生物質部會會員、上海市化學化工學會生物工程專業委員會會員。

團隊成員：胡佳俊，博士，講師。2012年博士畢業于同濟大學環境工程專業。2014年至今受聘于上海大學生命科學學院。主要研究方向為生物質利用、土壤修複及微生物固碳。發表學術論文五十餘篇，以第一或通訊作者發表SCI論文15篇；以第一作者出版學術專著1本；申請專利5項；主持國家自然科學基金青年項目、中國博士後科學基金特别資助、中國博士後科學基金面上資助、污染控制與資源化研究國家重點實驗室自主課題資助各一項。

主要研究領域

1. 從事生物資源·廢棄物的循環利用及其高效利用技術方面的研究。通過開發非糧食原料的預處理工藝，改進發酵工藝和回收工藝，建立節能、環保的生物燃料/材料生産工藝；探讨生物煉制工藝在重金屬污染土壤修複中的應用，建立重金屬零回田的生物循環工藝。

2. 稭稈資源化及綜合利用：木質纖維素水解液中酚酸類物質及單甯類物質對微生物生長和發酵的影響機理研究及其應用基礎研究。研究生物質的預處理、糖化對多酚、多糖結構、酚酸類物質生成的影響。開發相應的發酵工藝及有用物質回收工藝。

代表性研究成果

論文：

[1] Shi J., Wang Y., Wei H., Hu J., Gao M-T. Structure analysis of condensed tannin from rice straw and its inhibitory effect on Staphylococcus aureus. Industrial

Crops & Products. 2020, 145, 112130.

[2] Hou.R., Shi J., Ma X., Wei H., Hu J., Tsang Y.F., Gao M-T. Effect of phenolic acids derived from rice straw on Botrytis cinerea and infection on tomato. Waste and Biomass Valorization. In Press.

[3] Hou.R., Hu J.,Wang Y., Wei H., Gao M-T. Simultaneous production of cellulose and ferulic acid esterase by Penicillium decumbens with rice straw as the sole carbon source. Journal of Bioscience and Bioengineering. In Press.

[4] Ma X., Hu J., Wang X., Choi S., Zhang T-A., Tsang Y.F., Gao M-T. An integrated strategy for the utilization of rice straw: Production of plant growth promoter followed by ethanol fermentation. Process Safety and Environmental Protection.

2019, 129: 1-7.

[5] Cui S., Ma X., Wang X., Zhang T-A., Hu J., Tsang Y.F., Gao M-T. Phenolic acids derived from rice straw generate peroxides which reduce the viability of Staphylococcus aureus cells in biofilm. Industrial Crops & Products.2019,140, 111561.

[6] Ma X., Chen X., Wang X., Choi S., Zhang T-A., Hu J., Tsang Y.F., Gao M-T. Extraction of flavonoids from the saccharification of rice straw is an integrated process for straw utilization. Applied biochemistry and biotechnology. 2019.

[7] Hu J., Guo H., Wang X., Gao M.-t., Yao G., Tsang Y.F., Li J., Yan J., Zhang S. Utilization of the saccharification residue of rice straw in the preparation of biochar is a novel strategy for reducing CO 2 emissions. Science of the Total Environment, 2019, 650: 1141-1148.

[8] Wang X., Cui S., Hu J., Ma X., Zhang T.-A., Tsang Y.F., Li J., Gao M.-T. Saccharides in straw hydrolysate decrease cell membrane damage by phenolics by inducing the formation of extracellular matrix in yeast. Carbohydrate Polymers, 2019, 219: 414-422.

[9] Chen X., Wang X., Xue Y., Zhang T.-A., Hu J., Tsang Y.F., Gao M.-T. Tapping the bioactivity potential of residual stream from its pretreatments may be a green strategy for low-cost bioconversion of rice straw. Applied Biochemistry and Biotechnology, 2018, 186: 507-524.

[10] Chen X., Wang X., Xue Y., Zhang T.-A., Li Y., Hu J., Tsang Y.F., Zhang H., Gao M.-T. Influence of rice straw-derived dissolved organic matter on lactic acid fermentation by Rhizopus oryzae. Journal of Bioscience and Bioengineering, 2018, 125: 703-709.

[11] Hu, J.; Xue, Y.; Guo, H.; Gao, M.-t.; Li, J.; Zhang, S.; Tsang, Y. F. Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity. Bioresource Technology, 2017, 227: 247-255.

[12] Zheng, W.; Zheng, Q.; Xue, Y.; Hu, J.; Gao, M.-T. Influence of rice straw polyphenols on cellulase production by Trichoderma reesei. Journal of Bioscience and Bioengineering, 2017, 123: 731-738.

[13] Zheng, W.; Chen, X.; Xue, Y.; Hu, J.; Gao, M.-T.; Tsang, Y. F. The influence of soluble polysaccharides derived from rice straw upon cellulase production by Trichoderma reesei. Process Biochemistry, 2017, 61: 130-136.

[14] Xue, Y.; Wang, X.; Chen, X.; Hu, J.; Gao, M.-T.; Li, J. Effects of different cellulases on the release of phenolic acids from rice straw during saccharification. Bioresource Technology, 2017, 234: 208-216.

[15] Wang, X.; Tsang, Y. F.; Li, Y.; Ma, X.; Cui, S.; Zhang, T.-A.; Hu, J.; Gao, M.-T. Inhibitory effects of phenolic compounds of rice straw formed by saccharification during ethanol fermentation by Pichia stipitis. Bioresource Technology, 2017, 244: 1059-1067.

[16] Chen, X.; Xue, Y.; Hu, J.; Tsang, Y. F.; Gao, M.-T. Release of polyphenols is the major factor influencing the bioconversion of rice straw to lactic acid. Applied Biochemistry and Biotechnology, 2017, 183: 685-698.

[17] Gao, M.-T.; Chen, X.; Zheng, W.; Xu, Q.; Hu, J. Calcium and manganese affect ethanol fermentation by Pichia stipitis in cadmium-containing medium by inhibiting cadmium uptake. Civil, architecture and environmental engineering, volume 1. CRC Press/Balkema, 2017.

[18] Zheng, Q.; Zheng, W.; Chen, R.; Hu, J.; Li, W.; Zhang, A.; Zhang, J.; Gao, M.-T. Use of a thermoresponsive polymer in ethanol fermentation carried out in a cadmium-containing medium. Enzyme and Microbial Technology, 2016, 85: 25-31.

[19] Xu, Q.; Wu, M.; Hu, J.; Gao, M.-T. Effects of nitrogen sources and metal ions on ethanol fermentation with cadmium-containing medium. Journal of Basic Microbiology, 2016, 56: 26-35.

[20] Hu, J.; Xu, Q.; Wu, M.; Meng, X.; Song, R.; Gao, M. Improved ethanol production in the presence of cadmium ions by a Saccharomyces cerevisiae transformed with a novel cadmium-resistance gene dvcrp1. Environmental Technology, 2016, 37: 2945-2952.

[21] 張天翺, 鄭威, 胡佳俊, 高旻天. 稻稈發酵生産纖維素酶的基質預處理工藝研究. 工業微生物, 2018, 48: 7-13.

[22] 胡佳俊; 高旻天. 基于科學實驗探索的土壤學教學改革及實踐. 教育教學論壇, 2016: 252-253.

[23] 胡佳俊; 朱仁懿; 高旻天. Acremonium 纖維素酶在玉米芯糖化中的應用. 工業微生物, 2015: 1-6.

專利：

[1] 利用稭稈制備香草醛和 4-乙烯基愈創木酚的方法.

[2] 一種稭稈糖化殘渣制備的生物碳及其作為土壤改良劑的應用.

[3] 從稭稈中提取纖維素酶生産誘導劑的方法及其在稭稈糖化中的應用.

[4] 一種強化膜生物反應器污染物去除性能的方法.

[5] 從甜葉菊稭稈中提取黃酮的方法.

[6] 稭稈多酚-單糖聯産工藝.

[7] 利用稭稈浸提液提高微生物培養及發酵效率的方法.

[8] 一種利用溫敏聚合物螯合污染土壤中重金屬的方法.

[9] 含有重金屬螯合基的溫敏聚合物及其制備方法和應用.

技術标準：

[1] 稭稈多酚. Q/320411BNX001-2018.

[2] 植物酚酸. Q/320411BNX002-2019.

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